Figure 4.

Molecular Cloning of gif1-1 and Complementation Test.

(A) Two markers delineated a 49-kb region containing two annotated genes: GRMZM2G481211 and GRMZM2G180246.

(B) Expression of GRMZM2G481211 (left) and GRMZM2G180246 (right) in immature tassels (∼5 mm) of wild type and gif1-1. The columns are the mean value of expression level detected in three separate experiments, each with three technical replicates. Error bar show the sd.

(C) Genic lesions of gif1-1. Exons are represented by black boxes; UTRs are indicated by gray boxes; introns are indicated by thin black lines.

(D) Genic lesion of gif1-2; the CAG (Q92) codon is mutated to TAG (stop).

(E) Diagram of gif1 Mutator insertion sites.

(F) gif1 expression in the wild type (W22) and gif1-mum1.

(G) A gif1-mum1 plant during vegetative development.

(H) Excised mature leaf blades of gif1-mum1.

(I) Tassel phenotype of gif1-mum1.

(J) Ear phenotype of gif1-mum1.

(K) Tassel phenotypes of mutants in different inbred backgrounds.

(L) Tassels of gif1-mum3 in the family 2189.

(M) Tassels of +/gif1-mum3, gif1-2/gif1-mum2, and gif1-mum2/gif1-mum3 segregating in the family 2193.

(N) Tassels of +/gif1-mum3 and gif1-2/gif1-mum3 in the family 2192.