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. Author manuscript; available in PMC: 2018 Mar 26.
Published in final edited form as: Virology. 2008 Sep 10;380(2):264–275. doi: 10.1016/j.virol.2008.08.011

Fig. 8.

Fig. 8

Real-time RT-PCR analysis of RTA, ORF57 and ORF59 transcripts in TRE-BCBL1-TRE cells. The RTA-inducible BCBL-1 cells, infected with lentivirus carrying empty control pLL3.7 or ORF57 shRNA expression vectors, were treated with 5 μg/ml tetracycline and then harvested at various time points. Total RNA was extracted by TRIzol reagent. A total of 5 μg of RNA was used with the First Strand cDNA Synthesis Kit to construct cDNA. The real-time PCR was performed using the SYBR green real-time PCR master mix kit with β-actin as the standard. The relative quantitative comparison of RTA, ORF57 and ORF59 mRNA levels over the time course of induction of the TRE-BCBL1-RTA-Ctrl (A) and TRE-BCBL1-RTA-57shRNA (B) cells infected with lentivirus control or shRNA expression vectors respectively. The standard curves are shown to the right (C). Each time point was tested in triplicate for the calculation of the mean and standard deviation.