Table 2. L1 I and L1 II. Primers sequences and annealing temperatures.

Target Region	HPV type	PCR primers and assay conditions			Pyrosequencing primers
		PCR primer a	Ampl. bp	PCR ann. T	Sequencinga primer	HPV strand
L1 I b	16,31,33,35,52,58	Forward GATATTTGTAAAAAAATATGGAA Reverse bio-AATAACTTTTATTTACATCCTAATTAT	78	45	All types GATATTTGTAAAAAAATATGGAA	Lower
	56	Forward TGAAATAGGTGTTGGAGGTAGAT Reverse bio-CCCATAATATATATATACAAAAATCCTCCT	143	62	Type 56 GTTATTTGTAAAAAAATAGGGAA	Lower
	18	Forward TTTATATTGGTATGTAGAAATTTTAGG Reverse bio-CCCTATTTTTTTACAAATAACTTTATAAC	229	50	Type 18 TTATAGAAGGTGGTGGAAGAT	Lower
	39,45,51,59	Forward TTTATATTGATATGAGGATATTTTAGG Reverse bio- CCCTATTTTTTTACAAATAACTTTATAAC	229	50	Type 39 AAATATATTATGTTGTTATTAGAT Type 45 TTATAGAAGGTGGTGGAAGAT Type 51 ATATATTTTGTTGTTATTAGTG Type 59 GTAGATATATTTTGTTGTTATTAGA	Lower
L1 II c	16,33,35,52,56,58	Forward GTTAGATATTTTTTTAATAGGG Reverse bio-ATTACCCCAACAAATACCATTA	207–219	57	Type 16 TGTTGGTGAAAATGTATTAG Type 33 GGTATATTAGGAGAGGTTGT Type 35 ATATTTAATAAATTATATTGGTTGT Type 52 GTTAGATATTTTTTTAATAGGG Type 56 GAGAATTTTTTTTTAGTTTTGTATA Type 58 TAGGGTTGGAAAATTTGG	Upper
	31	Forward GATGTATAAATATTTAATAAATTA Reverse bio- ATTACCCCAACAAATACCATTA	75	45	Type 31 GATGTATAAATATTTAATAAATTA	Upper
	18,51	Forward GTTTGTAGATTTTTATGGGGATTTTATG Reverse bio- ACCAACAAATACCATTATTATAACCCT	266	50	Type 18 ATATATTAAAGGTATAGGTATG	Upper
	39,45,59	Forward TTGTAAGATATTTTTGGAAT Reverse bio- ACCAACAAATACCATTATTATAACCCT	210	55	Type 39 AATTGTATATTAAGGGTA Type 45 AGATTTATATATTAAAGGTATTAG Type 51 ATTATTATATTAAGGGTAGTGGTA Type 59 GTTATATATTAAAGGTATTGA	Upper

^a Bisulfite modified sequences

^b L1 I. HPV L1 gene. Reference HPV16 CpG: 5601, 5606, 5609, 5616

^c L1 II. HPV L1 gene. Reference HPV16 CpG: 6457