Fig 1. LC-MS/MS profiles of serum samples with BBR introduced in silico and in vivo.

Chromatograms of (A) blank serum (B) blank serum spiked with (1) d6-BBR (31.25 ng/mL) and (2) BBR (7.8 ng/mL), and (C) serum from a Sprague-Dawley rat treated with 90 mg/kg BBR TD and collected 0.5 hours post administration demonstrating the presence of internal control (1) d6-BBR, (2) BBR, and the glucuronide metabolite (3) DMG.