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. Author manuscript; available in PMC: 2018 Mar 26.
Published in final edited form as: Reproduction. 2016 Oct 10;153(1):1–10. doi: 10.1530/REP-16-0494

Figure 6. PAD inhibition decreases expression of IGFBP1 mRNA in OLE cells.

Figure 6

OLE cells were treated with vehicle (DMSO) or 2 μM BB-ClA for 3 and 6 hours. Total RNA was purified from OLE cells, reverse transcribed and resulting cDNA was examined by qPCR with intron spanning primers for IGFBP1, HSD11B1 or GAPDH as the reference gene control. Data was analyzed using the ΔΔct method where IGFBP1 and HSD11B1 ct values were normalized to GAPDH and are represented as fold change in mRNA expression. Data are expressed as means ± SEM. Means were separated using Student’s T-Test and *** indicating significant differences (P< 0.0001).