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. 2017 Dec 20;7(24):e2654. doi: 10.21769/BioProtoc.2654

Figure 5. Purified myonuclei.

Figure 5.

A. Nuclei isolated by ultracentrifugation through 2.1 M sucrose. Nuclei are visible in DAPI channel. B. Affinity purified nuclei isolated by binding of TMEM38A antibody and magnetic beads to nuclei. Nuclei are visible in DAPI channel. Scale bar = 10 µm.