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. 2018 Mar 1;145(5):dev149419. doi: 10.1242/dev.149419

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Persistence of HE core-like structures in doxycycline-induced iRunx1ko^{Cbfb^−/−} cultures. (A) Representative pictures of day 5 iRunx1ko (top) and iRunx1ko^{Cbfb^−/−} (bottom) EHT cultures. HE core-like structures (arrowheads) can be observed in doxycycline-induced iRunx1ko^{Cbfb^−/−}. (B) Flow cytometry analysis of day 5 iRunx1ko and iRunx1ko^{Cbfb^−/−} EHT cultures with or without doxycycline. Individual biological experiments and mean±s.d. (only for N>2) are shown. Two-way ANOVA (only for N>2). (C) Myeloid hematopoietic colony-formation assay with iRunx1ko and iRunx1ko^{Cbfb^−/−} re-plated after 3 days of EHT culture. Individual biological experiments and mean±s.d. (only for N>2) are shown. Two-way ANOVA (only for N>2). N=biological replicates.