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. 2018 Mar 1;145(5):dev151555. doi: 10.1242/dev.151555

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© 2018. Published by The Company of Biologists Ltd

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Fig. 6. — AKT1 and AKT3 are phosphorylated in different types of spermatogonia. (A) Whole-mount wild-type or Tg(Gdnf) tubules co-immunostained for phosphorylated AKT1 Ser473 (P-AKT1) and GFRA1. GFRA⁺ cells in wild-type testes (arrows) and large clusters of GFRA⁺ cells in Tg(GDNF) testes (asterisks) are P-AKT1 negative. (B) Whole-mount wild-type or Tg(Gdnf) tubules co-immunostained for phosphorylated AKT3 Ser472 (P-AKT3) and GFRA1. Arrows indicate GFRA1⁺ P-AKT3⁺ A_s and A_pr spermatogonia. Arrowheads indicate GFRA1⁺ P-AKT3⁺ cluster in Tg(Gdnf) tubules. See also Figs S5 and S6. Scale bars: 100 µm.