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. 2018 Mar 1;145(5):dev151555. doi: 10.1242/dev.151555

Fig. 7.

Fig. 7.

Effect of germ cell-specific deletion of Akt1 on germ cell development. (A-C) No significant change in body weight (A), testis weight (B) or sperm count (C) in 2-month-old mice when Akt1 is deleted in germ cells (n=3). (D) Paraffin wax-embedded sections of testis from control and mutant testis stained with PSA and probed with PLZF antibody. (E) Average PLZF+ cells per tubule (200-250 tubules counted) in control and mutant testis (n=3). (F) Akt1 transcript is significantly (***P=0.002) downregulated in mutant testis (n=3) as determined by qRT-PCR. (G) Absence of P-AKT1 staining in the mutant germ cells. Arrows indicate GFRA1+ P-AKT1+ (double-positive) cells. (H) Detection of P-AKT3 in Akt1-deleted germ cells. Arrows indicate GFRA1+ P-AKT3+ (double-positive) cells. Scale bars: 100 µm.