Fig. 8.

BCI augments NRG1 activity in primary rat cardiomyocytes. Postnatal day 1 rat neonatal ventricular myocytes were isolated and cultured in 10% FBS. Cells were exposed to DMSO vehicle (A), NRG1 (50 ng/ml) (B) or NRG1 plus BCI at 5 µM (C) for 3 days. All treatments were performed in the presence of 10% FBS. Cardiomyocyte proliferation was quantified by H3P staining (n=2). (D) BCI treatment augments the effect of NRG1 on neonatal cardiomyocyte proliferation. ****P<0.0001, ***P<0.001, ANOVA followed by Bonferroni post-hoc test.