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. 2018 Feb 15;145(4):dev155275. doi: 10.1242/dev.155275

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Cx30 regulates hippocampal synaptic levels of laminin proteins. (A) Western blot detection of pre- (synapsin I, SYN1) and post- (PSD95) synaptic proteins showed an enrichment of plasma membrane synaptic proteins in crude synaptosomal membrane fraction (S) compared with total hippocampal fraction (T), whereas actin protein levels were clearly similar in both fractions. (B) Immunoblot detection of laminin expression in synaptosomal membrane fractions from mouse hippocampi. (C) Histogram showing the quantitative analysis of normalized laminin expression levels (+/+, n=3 mice; −/−, n=3 mice). The loss of Cx30 expression increased significantly the synaptic levels of laminin. (D) Immunoblot detection of laminin β2 protein levels in crude hippocampal membrane fractions. (E) Quantitative analysis of laminin β2 normalized expressions. Hippocampal synapses lacking Cx30 upregulated laminin β2 expression. In both experiments, actin was used as a loading control. Asterisks indicate statistical significance (*P<0.05). S+/+, wild-type mice; S−/−, Cx30^−/− mice.