(A, C) Behavior ethograms upon optogenetic stimulation of 412-Gal4 or class IV neurons. Groups of animals expressing ReaChR in either population were subjected to optogenetic activation at different light intensities for 10 s: Lowest (~45 Lux), Low (~200. Lux), Moderate (~850 Lux) and Highest (~1450 Lux). Behavior events are color-coded: crawling (grey), pause (blue), bending (green), and rolling (orange). (A) Behaviors triggered upon optogenetic activation of class IV neurons. Lowest, n = 24. Low, n = 26 Moderate, n = 20; Highest, n = 23. (B) Behaviors triggered upon optogenetic activation of 412-Gal4 neurons. Lowest, n = 22. Low, n = 30; Moderate, n = 26; Highest, n = 30. (C) Percent of larvae exhibiting crawling (grey), pausing (blue), rolling (orange) and bending (green) across different activation intensities. (See also Figure 2E).
Figure 2—source data 1. Summary table of behavioral responses to dose-dependent optogenetic activation.Number of animals displaying bending, rolling, pausing, or crawling during optogenetic activation of ppk1.9-Gal4 or 412-Gal4 neurons across different levels of activation.