Skip to main content
. 2018 Mar 26;7:e32510. doi: 10.7554/eLife.32510

Figure 4. Light-induced pause in CINs decreases intrinsic excitability of SPNs via M1 receptor signaling.

Figure 4.

Representative traces of an SPN showing membrane responses to current injections before (black) during (orange) and after a pause (gray). (B, E) Pooled data showing average rheobase current. Before vs pause: p =<0.0001; pause vs after: p =<0.0001, N/n = 8/12 in (B), by Tukey's multiple comparisons test. Before vs pause: p =<0.0001; before vs pirenzepine: p=0.0005; before vs pirenzepine + light: p=0.0001; n = 7 in (E), by Tukey's multiple comparisons test. (C, F) Action potential numbers plotted against current injection (N/n = 8/12 in C; N/n = 6/7 in F). (D) Representative traces of an SPN showing membrane responses under different conditions. (G) Tonic level of acetylcholine control SPNs excitability via activation of muscarinic receptors (1). Light-induced pause lowers acetylcholine (ACh) levels reducing M1 activation and decreasing excitability of SPNs (2). M1 receptors selective antagonist pirenzepine decreases SPNs excitability (3). Pirenzepine occludes the effect of the pause (4). In (C) and (F) R represents the minimum current needed to trigger an action potential in basal conditions. Data are represented as mean ± SEM; ns = not significant. N = mice; n = cells.

Figure 4—source data 1. This spreadsheet contains the intrinsic properties and firing responses for the individual neurons shown in Figure 4.
elife-32510-fig4-data1.xlsx (17.6KB, xlsx)
DOI: 10.7554/eLife.32510.016