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GCaMP6m was used as a proxy to explore the effect of disrupted Nlg1 signalling on late-stage postsynaptic activity. In controls, calcium activity within 10 hr prior to eclosion is characterised by changes in fluorescence which are synchronous across the muscle field (Mef2-GAL4 > GCaMP6m). Frames recorded at 1 s intervals. Time format mm:ss.
