Video 7. Nlg1::GFP puncta are correlated with filopodia stability and growth.

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DOI: 10.7554/eLife.31659.014

Nlg1::GFP (green) in muscles forms puncta which decorate the axon terminals (magenta), particularly at sites of filopodia growth and at the tips of filopodia (38 hr APF; Mef2-GAL4 > Nlg1::GFP, VGlut-LexA > myr::tdTomato). Filopodia which are tipped with stable Nlg1::GFP puncta are significantly longer lived and less motile than those which are not. Filopodia retract only to the point of the last stable Nlg1::GFP punctum. Frames taken at 1 min intervals. Time format: hh:mm.