Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| genetic reagent (D. melanogaster) | OK371-GAL4 | Bloomington Drosophila Stock Centre | BDSC:26160 | |
| genetic reagent (D. melanogaster) | Mef2-GAL4 | Bloomington Drosophila Stock Centre | BDSC:27390 | |
| genetic reagent (D. melanogaster) | PPK-GAL4 | Bloomington Drosophila Stock Centre | BDSC:32078 | |
| genetic reagent (D. melanogaster) | VGlutNMJX-GAL4 | Daniels et al. (2008) | P{VGlut-GAL4.D}NMJX | |
| genetic reagent (D. melanogaster) | VGlut(Trojan)-T2A-LexA | Bloomington Drosophila Stock Centre | BDSC:60314 | |
| genetic reagent (D. melanogaster) | Df(2L)vglut2 | Daniels et al. (2006) | ||
| genetic reagent (D. melanogaster) | Nlg1I960 | Banovic et al. (2010) | ||
| genetic reagent (D. melanogaster) | Nlg1ex2.3 | Banovic et al. (2010) | ||
| genetic reagent (D. melanogaster) | Nrx241 | Li et al. (2007) | ||
| genetic reagent (D. melanogaster) | Df(3R)BSC747 | Gift from Hermann Aberle | FlyBase: FBab0045813 | |
| genetic reagent (D. melanogaster) | Df(3R)Exel6191 | Gift from Hermann Aberle | FlyBase: FBab0038246 | |
| genetic reagent (D. melanogaster) | RN2-Flp | Roy et al. (2007) | ||
| genetic reagent (D. melanogaster) | UAS-myr::GFP | Pfeiffer et al., 2012 | ||
| genetic reagent (D. melanogaster) | UAS-cytoplasmicGFP | Pfeiffer et al., 2012 | ||
| genetic reagent (D. melanogaster) | UAS-Lifeact::Ruby | Bloomington Drosophila Stock Centre | BDSC:35545 | |
| genetic reagent (D. melanogaster) | UAS-CLIP170::GFP | Stramer et al., 2010 | ||
| genetic reagent (D. melanogaster) | UAS-mCD8::ChRFP | Bloomington Drosophila Stock Centre | BDSC:27391 | |
| genetic reagent (D. melanogaster) | UAS-myr::tdTomato | Bloomington Drosophila Stock Centre | BDSC:30124 | |
| genetic reagent (D. melanogaster) | UAS-BRP::RFP | Gift from Stephan Sigrist | ||
| genetic reagent (D. melanogaster) | UAS-Nlg1::GFP | Banovic et al. (2010) | ||
| genetic reagent (D. melanogaster) | UAS-Nlg1untagged | Banovic et al. (2010) | ||
| genetic reagent (D. melanogaster) | UAS-Nrx::GFP | Banovic et al. (2010) | ||
| genetic reagent (D. melanogaster) | UAS-Liprin-α::GFP | Fouquet et al. (2009) | ||
| genetic reagent (D. melanogaster) | UAS-Syd1::Straw | Owald et al. (2010) | ||
| genetic reagent (D. melanogaster) | UAS-GCaMP6M | Bloomington Drosophila Stock Centre | BDSC: 42750 | |
| genetic reagent (D. melanogaster) | UAS-GCaMP6M | Bloomington Drosophila Stock Centre | BDSC: 42748 | |
| genetic reagent (D. melanogaster) | GluRIIE::GFP | FlyFos TransgeneOme (fTRG) library | fTRG:154 | |
| genetic reagent (D. melanogaster) | BRP::GFP | Bloomington Drosophila Stock Centre | BDSC:59411 | |
| genetic reagent (D. melanogaster) | VGlut::GFP | Bloomington Drosophila Stock Centre | BDSC:59292 | |
| genetic reagent (D. melanogaster) | Syt1::GFP | Bloomington Drosophila Stock Centre | BDSC:59788 | |
| genetic reagent (D. melanogaster) | Nlg1FlpStop | FlpStop construct from Fisher et al. (2017) |
||
| genetic reagent (D. melanogaster) | LexAop-myrGFP | Bloomington Drosophila Stock Centre | BDSC:32210 | |
| antibody | antiVglut (C-term) (rabbit polyclonal) |
Mahr and Aberle (2006) | RRID:AB_2490071; aa 561–632 |
(1:10,000) |
| antibody | antiBRP (nc82) (mouse monoclonal) |
Developmental Studies Hybridoma Bank |
RRID:AB_231486; aa 1227–1740 |
(1:5) |
| antibody | antiGFP (chicken polyclonal) |
Thermo Fisher Scientific (Invitrogen) | Cat# PA1-86341, RRID:AB_931091 |
(1:1000) |
| antibody | antiHRP-Cy3 (goat polyclonal) |
Jackson Immunoresearch | (1:5000) | |
| antibody | Alexa-488 secondary | Thermo Fisher Scientific (Invitrogen) | (1:500) | |
| antibody | Cy3 and Cy5 secondaries | Jackson Immunoresearch | (1:500) |