Table 1.
Effect of pharmacological manipulation of MPOA CRFR1 or CRFR2 on non-maternal behaviors under non-stress conditions on LD 1.
| Behavior | Group | Occurrence [n] |
||||||
|---|---|---|---|---|---|---|---|---|
| Basal | 0 min | +30 min | +60 min | +90 min | +300 min | +330 min | ||
| Off-nest | VEH | 1.1 ± 0.5 | 1.6 ± 0.8 | 0.1 ± 0.1 | 2.1 ± 1.2 | 1.7 ± 1.5 | 0.9 ± 0.5 | 2.7 ± 2.0 |
| CRFR1 ago | 1.5 ± 0.7 | 11.8 ± 1.0**++ | 7.8 ± 2.3** | 9.7 ± 2.6** | 8.8 ± 2.8** | 7.8 ± 2.7** | 7.8 ± 2.2 | |
| CRFR1 ant | 1.3 ± 0.4 | 3.3 ± 1.5 | 0.8 ± 0.6 | 1.8 ± 0.8 | 0.5 ± 0.3 | 1.2 ± 0.6 | 1.3 ± 0.8 | |
| CRFR2 ago | 0.7 ± 0.2 | 8.9 ± 2.0**++ | 6.0 ± 2.5* | 1.4 ± 0.6 | 0.9 ± 0.3 | 2.9 ± 2.0 | 2.6 ± 1.8 | |
| CRFR2 ant | 1.0 ± 0.5 | 4.7 ± 2.2+ | 1.4 ± 0.6 | 1.1 ± 1.0 | 0.1 ± 0.1 | 0.1 ± 0.1 | 3.6 ± 1.8 | |
| Locomotion | VEH | 0.6 ± 0.4 | 1.0 ± 0.6 | 0.0 ± 0.0 | 0.9 ± 0.4 | 1.0 ± 0.8 | 0.4 ± 0.3 | 0.3 ± 0.2 |
| CRFR1 ago | 0.2 ± 0.1 | 7.3 ± 1.1**++ | 4.5 ± 1.6** | 6.5 ± 1.6** | 5.8 ± 2.1** | 5.8 ± 2.5** | 6.5 ± 2.0** | |
| CRFR1 ant | 0.7 ± 0.3 | 2.5 ± 1.3 | 0.2 ± 0.2 | 1.2 ± 0.4 | 0.2 ± 0.2 | 0.2 ± 0.2 | 0.8 ± 0.3 | |
| CRFR2 ago | 0.4 ± 0.1 | 2.8 ± 0.7+ | 1.4 ± 0.7 | 0.5 ± 0.3 | 0.9 ± 0.3 | 0.0 ± 0.0 | 0.1 ± 0.1 | |
| CRFR2 ant | 0.6 ± 0.3 | 1.4 ± 0.7 | 0.4 ± 0.3 | 0.9 ± 0.7 | 0.1 ± 0.1 | 0.1 ± 0.1 | 1.0 ± 0.4 | |
| Self-grooming | VEH | 0.3 ± 0.1 | 0.4 ± 0.3 | 0.1 ± 0.1 | 0.4 ± 0.3 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.3 ± 0.2 |
| CRFR1 ago | 0.2 ± 0.1 | 3.5 ± 0.8**++ | 2.7 ± 0.5** | 1.7 ± 0.6** | 1.1 ± 0.5** | 0.5 ± 0.2 | 0.5 ± 0.5 | |
| CRFR1 ant | 0.6 ± 0.2 | 0.6 ± 0.3 | 0.5 ± 0.3 | 0.3 ± 0.2 | 0.3 ± 0.3 | 0.3 ± 0.3 | 0.0 ± 0.0 | |
| CRFR2 ago | 0.2 ± 0.1 | 1.4 ± 0.4++ | 0.3 ± 0.2 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.3 ± 0.2 | 0.5 ± 0.3 | |
| CRFR2 ant | 0.1 ± 0.1 | 0.7 ± 0.4 | 0.3 ± 0.2 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 1.0 ± 0.5 | |
| Sleeping/ resting |
VEH | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.4 ± 0.4 | 0.0 ± 0.0 | 0.4 ± 0.4 | 2.1 ± 2.1 |
| CRFR1 ago | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.2 ± 0.2 | 0.8 ± 0.8 | 0.0 ± 0.0 | 0.0 ± 0.0 | |
| CRFR1 ant | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.7 ± 0.7 | 0.0 ± 0.0 | |
| CRFR2 ago | 0.0 ± 0.0 | 4.6 ± 2.0 | 4.4 ± 2.2 | 0.6 ± 0.6 | 0.0 ± 0.0 | 2.6 ± 1.8 | 1.9 ± 1.9 | |
| CRFR2 ant | 0.0 ± 0.0 | 2.3 ± 1.5 | 0.6 ± 0.6 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.6 ± 0.6 | |
The occurrence of all off-nest behaviors was scored for 60 min before (averaged as basal) and 90 min after drug infusion (immediately before t 0 min), as well as during an additional 60 min period in the afternoon. Off-nest behavior is further divided into locomotion (including digging/burrowing and any explorative behavior in the home cage), self-grooming, and sleeping/resting. For details on treatments, see the Fig. 2 legend. Data are presented as group means ± SEM. n = 6–8 rats per group. **p ≤ 0.01, *p ≤ 0.05 versus VEH; ++ p ≤ 0.01, + p ≤ 0.05 versus basal.