Table 2.

maM35c4 mutations.

MERS region/protein	Type of mutation in maM35c4 clone	Amino acid position	Nucleotide change	Nucleotide numbering
5′ UTR	single nucleotide change	N/A	CTCACTT → CTCGCTT	28
nsp3	Missense	A217V	GCA → GTA	3486–3488
nsp3	Missense	T1615N	ACT → AAT	7680–7682
nsp6	Missense	L232F	CTT → TTT	11631–11633
nsp8a	Missense	I108L	ATT → CTT	12384–12386
nsp14	Missense	T521I	ACA → ATA	19562–19564
spike	Missense	N222Y	AAT → TAT	22119–22121
spikea	Insertion of RMR	R884-RMR-S885L	AATGCGGCT	24109||24110
spikea	Missense	S885L (L is 888 in new sequence)	AGT → CTT	24108–24110
Orf3	Nonsense	Q4stop	CAA → TAA	25541–25543
Orf4a	Missense	P85L	CCT → CTT	26104–26106
Orf4b/Orf5	Large deletion between Orf4b & Orf5	N/A	GATTTT…Δ…AAACCC	26211–26863
Membrane	Missense	S2F	TCT → TTT	27856–27858

Protein and nucleotide sequence numbering is based on the EMC 2012 sequence (JX869059.2) with nonstructural protein annotations described in NC_019843

Numbering for the amino acids begins with the first amino acid of each individual protein.

^aMutations that can also be found in icMERSma1 (Cockrell et al., 2016).