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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Int J Antimicrob Agents. 2017 Dec 27;51(4):586–593. doi: 10.1016/j.ijantimicag.2017.12.016

Fig. 1.

Fig. 1

(A) Multiple sequence alignment (MSA) of different PEA transferases selected from representative strains of E. coli, including MCR-1, MCR-2, EptA, EptB and EptC. The residues are coloured by their degree of conservation. The darker the colour is, the more conserved the amino acid is. Black lines underneath the alignment represent conserved motifs among MCR-1, MCR-2 and Ec_EptA but not homologous among Ec_EptB or Ec_EptC; while cyan lines under the alignment represent homologous motifs among MCR-1, MCR-2, Ec_EptA and Ec_EptB but not in Ec_EptC. Abbreviations and relevant accession numbers listed in Fig. 1 are as follows: MCR-1 (from the plasmid in E. coli SHP45, WP_049589868.1); MCR-2 (from the plasmid in E. coli KP37, WP_065419574.1); Ec_EptA (in E. coli K-12 MG1655, NP_418538.2); Ec_EptB (in E. coli K-12 MG1655, NP_418002.2) and Ec_EptC (in E. coli K-12 MG1655, NP_418390.1). (B) Heatmap of pairwise identities among MCR-1, MCR-2, EptA, EptB and EptC based on the sequence BLAST.