Table 3.
Substrate | ||||
---|---|---|---|---|
| ||||
Carbon source/Strain | NADH | NADH/CN− | Asc-TMPD | Asc-TMPD/CN− |
Glu/KF707 W.T. | 158 ± 9,0 | 31 ± 5,0 | 150 ± 15 | 10 ± 2,0 |
Glu/KFΔc4 | 67 ± 2,0 | 55 ± 4,0 | 52 ± 2,0 | 4,5 ± 1,0 |
Glu/KFΔcco1-2 | 160 ± 8,0 | 114 ± 0,1 | 38 ± 1,0 | 8,0 ± 1,0 |
Glu/KFΔc4/cco1-2 | 85 ± 6,0 | 80 ± 5,0 | 10 ± 1,0 | 8,0 ± 1,0 |
| ||||
Bph/KF707 W.T. | 110 ± 3,0 | 10 ± 0,5 | 161 ± 12 | 8,0 ± 2,0 |
Bph/KFΔc4 | 34 ± 2,0 | 31 ± 2,0 | 32 ± 2,0 | 3,0 ± 1,0 |
Bph/KFΔcco1-2 | 97 ± 8,0 | 27 ± 1,5 | 115 ± 11 | 5,0 ± 1,5 |
Bph/KFΔc4/cco1-2 | 28 ± 1,5 | 27 ± 1,5 | 15 ± 1,0 | 5,3 ± 1,0 |
Non standard abbreviations used: Asc, sodium ascorbate (5 mM); TMPD, N,N,N′,N′-tetramethyl-p-phenylenediamine (50 μM); CN−, potassium cyanide (50 μM). Mutant strains: KFΔc4, lacks cytochrome c4; KFΔcco1-2, lacks both cbb31- and cbb32-Cox; KFΔc4/Δcco1-2, lacks cytochrome c4 and the cbb3-1- and cbb32-Cox. Oxygen consumption rates are expressed as nmoles of O2 consumed min−1 mg of protein−1, are the mean values of at least two to three assays.