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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: FEBS Lett. 2018 Mar 1;592(6):901–915. doi: 10.1002/1873-3468.13001

Table 3.

Respiratory activities in KF707 membranes from W.T. and appropriate mutant cells grown until the stationary phase (OD600 nm 0.7–0.9) in glucose (Glu) or in biphenyl (Bph) as the sole carbon source. See the text for further details.

Substrate

Carbon source/Strain NADH NADH/CN Asc-TMPD Asc-TMPD/CN
Glu/KF707 W.T. 158 ± 9,0 31 ± 5,0 150 ± 15 10 ± 2,0
Glu/KFΔc4 67 ± 2,0 55 ± 4,0 52 ± 2,0 4,5 ± 1,0
Glu/KFΔcco1-2 160 ± 8,0 114 ± 0,1 38 ± 1,0 8,0 ± 1,0
Glu/KFΔc4/cco1-2 85 ± 6,0 80 ± 5,0 10 ± 1,0 8,0 ± 1,0

Bph/KF707 W.T. 110 ± 3,0 10 ± 0,5 161 ± 12 8,0 ± 2,0
Bph/KFΔc4 34 ± 2,0 31 ± 2,0 32 ± 2,0 3,0 ± 1,0
Bph/KFΔcco1-2 97 ± 8,0 27 ± 1,5 115 ± 11 5,0 ± 1,5
Bph/KFΔc4/cco1-2 28 ± 1,5 27 ± 1,5 15 ± 1,0 5,3 ± 1,0

Non standard abbreviations used: Asc, sodium ascorbate (5 mM); TMPD, N,N,N′,N′-tetramethyl-p-phenylenediamine (50 μM); CN, potassium cyanide (50 μM). Mutant strains: KFΔc4, lacks cytochrome c4; KFΔcco1-2, lacks both cbb31- and cbb32-Cox; KFΔc4/Δcco1-2, lacks cytochrome c4 and the cbb3-1- and cbb32-Cox. Oxygen consumption rates are expressed as nmoles of O2 consumed min−1 mg of protein−1, are the mean values of at least two to three assays.