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. 2018 Mar 20;9:246. doi: 10.3389/fphys.2018.00246

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Copyright © 2018 Querio, Antoniotti, Foglietta, Bertea, Canaparo, Gallo and Levi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chamazulene and ROS levels in flow cytometry after HG treatment. (A) Histograms illustrate the relative MFI toward control derived from flow cytometry and show ROS levels when cells are treated with HG and their decrease in a simultaneous treatment with Cham and HG. Cham effect is not affected by ethanol. Data shown are mean ± SD of three independent experiments (HG: 1.47 ± 0.35; Cham: 0.94 ± 0.03; HG + Cham: 0.96 ± 0.02; EtOH: 1.08 ± 0.02); ^*P < 0.05 (B) Representative graphs of flow cytometry in which is illustrated the variation in fluorescence in different treatments.