FIGURE 1.

Decreased β-arrestin 1 expression in HET mice and negligible β-arrestin 1 expression in KO mice. Compared to WT, decreased β-arrestin 1 expression was observed via Western blot of cerebellar protein extracts in HET and KO β-arrestin 1 mice (A), as normalized by α-tubulin expression and adjusted for background (B). A decrease in body weight was observed in β-arrestin 1 KO male (C) and female (D) mice. Significance by one-way ANOVA (β-arrestin 1 Western blot) or two-way ANOVA (body weight over time) with multiple comparisons (Tukey), ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001; data represented as mean ± SEM.