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. 2018 Mar 20;10:73. doi: 10.3389/fnagi.2018.00073

FIGURE 5.

FIGURE 5

2K1C increased levels Aβ, amyloid depositions and microglial activation in the hippocampal of 3xTg mice. 2-, 5-, and 7-month-old mice were given the 2K1C or the Sham operation, and their hippocampi were removed for analyses. (A) Hippocampal concentrations of soluble Aβ40 and Aβ42 at 1 month post-operation (termed 2 + 1, 5 + 1, and 7 + 1 groups). N = 2 + 1: Sham: 8, 2K1C: 3; 5 + 1: Sham: 3, 2K1C: 3; 7 + 1: Sham: 6, 2K1C: 4. (B) Thioflavin-S-positive amyloid plaques in the hippocampi of 7 + 3 groups. Thioflavin-S staining micrographs are shown on the left panels; the quantitative results are shown on the right panels. N = Sham: 5, 2K1C: 5. ∗p < 0.05, ∗∗p < 0.01 versus respective Sham group, two-tailed t-test. (C) Representative Iba1 immunostaining micrographs are shown on the left panels. The densities of Iba-1-positive microglial cells are shown on the right panel. N = 6 for each group. ∗∗p < 0.01, ∗∗∗p < 0.001 versus respective Sham group, Bonferroni’s post hoc test. (D) Expression of Iba-1-positive microglial cells in the hippocampal subregions of the 7 + 3 mice. N = 6 for each group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 versus respective Sham group, two-tailed t-test. D. Hippo, dorsal part of hippocampus; V. Hippo, ventral part of hippocampus; Post. CA, posterior CA part of hippocampus.