Figure 1.

Hyperthermia prevents IL-1f3 induced IκBCC phosphorylation and degradation. Shown in the upper section of this figure are data demonstrating that stimulation of FLS results in complete degradation of IκBCC within 15 minutes. However, prior exposure to heat shock for 30 minutes prevents this event irrespective of a recovery period of up to 2 hours at 37°C prior to addition of IL-1f3 for 15 minutes. As shown in the lower section, IκBCC phosphorylation is readily detectable in non heat-treated cells but absent in cells that were exposed to hyperthermia prior to IL-1p treatment. As indicated, IκBα phosphorylation was monitored after 1 and 3 hours of recovery (REC) at 37°C.