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. 2018 Mar 20;9:498. doi: 10.3389/fmicb.2018.00498

FIGURE 4.

FIGURE 4

Inhibition of RIP2 impairs OMVs-induced NF-κB activation in Caco-2 cells. Activation of NF-κB was assayed by means of IκBα degradation. (A) Caco-2 cells were stimulated with EcN or ECOR12 OMVs (10 μg/ml) for 20, 40, 90, 120, 150 and 180 min, and IκBα levels were assessed by Western blot. For normalization, blots were probed with an anti-NEK-9 antibody. For comparison, parallel stimulations were performed in Caco-2 cells treated with the RIP2 inhibitor Genfitinib (1 μM) (B), transfected with si-NOD1 (C), or transfected with si-NOD2 (D). Densitometric quantification of IκBα was performed with samples from three independent experiments. Representative immunoblots were shown. Normalized values from untreated control cells (time 0 min) were set as 1. Significance against untreated control cells (∗p ≤ 0.05).