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Time course of translocation of fragmented tRNAMet incorporating 2′-hydroxyl group substitutions within the small fragment. Lanes are as described above in Fig. 2. Ligated, tRNAMet fragment 1–56 ligated to the respective small fragment 57–76. 2′-F66 and 2′-F71 are 2′-fluoro substitutions at positions 66 and 71, respectively; 2′-N66 and 2′-N71 are 2′-amino substitutions at positions 66 and 71, respectively.
