Figure 4.
Kinetic analysis of translocation of fragmented tRNAMet incorporating 2′-hydroxyl group substitutions. (A) Translocation of 2′-O-methyl substituted tRNAMet. (B) Translocation of 2′-deoxy substituted tRNAMet. (C) Translocation of ligated tRNAMet incorporating 2′-deoxy substitutions. (D) Translocation of 2′-fluoro and 2′-amino substituted tRNAMet. Labels are as described above in Figs. 2 and 3. Values are normalized with respect to translocation of control tRNAMet done in parallel. Results are the average of at least three experiments.