Table 2.

Primers used for PCR amplification of selected domains of Cry toxins with restriction sites underlined and melting temperatures (T_m) in °C.

Primer	Sequence (5′-3′)	Restriction Enzyme	Tm
1AcFD1	TGGAGGTCATATGGATAACAATCCGAACATC	NdeI	69.7
1AcRD2	GTAGTCGACTTCAGCACTACGATGTATCCA	SalI	70.8
2AcFD3	TCTGTCGACTTCACCGTATCTCCAATACATGCC	SalI	73.0
2AcRD3	TGTCTCGAGTTAATACAGTGGTGGAAGGTTAG	XhoI	71.3