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. 2018 Feb 28;10(3):98. doi: 10.3390/toxins10030098

Figure 2.

Figure 2

ZEA modulates expression of VIM, ZEB1, ZEB2 and TGFβ1 dependent on ERα expression. (A) the relative expression of VIM; (B) the relative expression of ZEB1; (C) the relative expression of ZEB2; (D) the relative expression of TGFβ1. The relative expression of genes are the results from RTqPCR calculated as the ratio of ΔΔCt calculated expression of gene of interest and reference genes RPLP0, RPS17 and H3F3A and are expressed as mean ± SE. Statistically significant results were marked with lines, * p < 0.05, ** p < 0.01, *** p < 0.001. RPLP0—60S acidic ribosomal protein P0, RPS17—40S ribosomal protein S17, H3F3A—Histone H3.3, VIM—vimentin, ZEB1—Zinc finger E-box-binding homeobox 1, ZEB2—Zinc finger E-box-binding homeobox 2, TGFβ1—Transforming growth factor beta 1, MPP—ERα antagonist, PHTPP—ERβ antagonist, ZEA—zearalenone, Cnt—control.