Table 3.
Overview of conditions and yields of different glucosylation batches. 15-Ac-DON, 15-acetyl-deoxynivalenol; HT2, HT-2 toxin; T2 triol, T-2 triol; FUSX, fusarenon X; T2, T-2 toxin, DAS, 4,15-diacetoxyscirpenol; NEO, neosolaniol; Tris, tris(hydroxymethyl)aminomethane buffer; KPP, potassium phosphate buffer; UGT, UDP-glucosyltransferase.
| 15-Ac-DON | HT2 | HT2 | T2 triol | FUSX | T2 | DAS | NEO | |
|---|---|---|---|---|---|---|---|---|
| Total amount of toxin (mg) | 10 | 5 | 5 | 2.2 | 6 | 5 | 5 | 6 |
| Concentration toxin (mM) | 1.5 | 0.6 | 0.6 | 1.15 | 2.1 | 2.8 | 2.7 | 2.8 |
| UDP-glucose (mM) | 2.3 | 1 | 1.2 | 10 | 10 | 10 | 10 | 10 |
| Buffer (100 mM, pH 7) | Tris | Tris | Tris | Tris | KPP | KPP | KPP | KPP |
| Temperature (°C) | 25 | 25 | 25 | 37 | 25 | 25 | 25 | 25 |
| Reaction time (h) | 24 | 24 | 4 | 16 | 72 | 72 | 72 | 72 |
| UGT (mg/mL) | 1 (A) | 1 (A) | 1 (A) | 2 (A) | 1.7 (B) | 1.7 (B) | 3.2 (B) | 4 (B) |
| Sucrose (mM) | 50 | 50 | 50 | 50 | ||||
| Sucrose synthase (mg/mL) | 0.9 | 0.9 | 1.8 | 1.8 | ||||
| Final amount glucoside (mg) | not stable | 5.6 (C) | 7.0 | 1.9 | 2.2 | 3.4 | 5.9 | 1.5/0.4 (D) |
(A)—OsUGT79, (B)—HvUGT13248, (C)—Degraded to T-2 tetraol-3-O-β-d-glucoside during NMR, (D)—Neosolaniol-3-O-β-d-glucoside/iso-neosolaniol-3-O-β-d-glucoside.