Table 6.
Primer sequences used in the quantitative PCR assays.
| Gene Name | Accession Number | Sequence 5′ to 3′ | Product Size (bp) |
|---|---|---|---|
| IFN-γ | NM_213948 | F: GGTAGCTCTGGGAAACTGAATG R: CTGACTTCTCTTCCGCTTTCTT |
150 |
| IL6 | NM_214399 | F: AGACGGATGCTTCCAATCTG R: CAGCCTCGACATTTCCCTTAT |
134 |
| IL10 | NM_214041 | F: ATCAAGGAGCACGTGAACTC R: CCTCTCTTGGAGCTTGCTAAA |
144 |
| IL12B | NM_214013 | F: GAAGTACAGAGTGGAGTGTCAG R: TGATGAAGAAGCTGCTGGTATAG |
128 |
| TNF-α | NM_214022 | F: CCTACTGCACTTCGAGGTTATC R: GGCTTTGACATTGGCTACAAC |
145 |
| PTGS2 | NM_214321 | F: GATGGCCACGAGTACAACTATC R: AAGATTCCTACCACCAGCAAC |
129 |
| CLDN3 | NM_001160075 | F: TCATCGGCAGCAGCATTATC R: GAGAGTCGTACACTTTGCACTG |
105 |
| CXCL10 | NM_001008691 | F: CAAGGAATACCTCTCTCCAGAAC R: ATCTCAACATGTGGGCAAGA |
128 |
| CCL4 | NM_213779 | F: TCCTGCTGCTTCACATACAC R: TACTCCTGGACCCAGTCATC |
158 |
| CCL2 | NM_214214 | F: TCACCTGCTGCTATACACTTAC R: GGTTCTGCACAGATCTCCTT |
139 |
| GAPDH | AF017079 | F: GTCTGGAGAAACCTGCCAAATA R: CCCAGCATCAAAGGTAGAAGAG |
152 |
Abbreviations: CCL: chemokine (C–C motif) ligand; CLDN3: claudin 3; CXCL10: C–X–C motif chemokine ligand 10; F: forward primer, GAPDH: glyceraldehyde 3-phosphate; IFN-γ: interferon gamma; IL: interleukin; TNF-α: tumor necrosis factor; PTGS2: prostaglandin-endoperoxide synthase 2; R: reverse primer.