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Deletion strategy and confirmation of the mutants used in this study. (A) Deletion and complement strategy for ∆gpaB strains; (B) Diagnostic PCR was performed to confirm the gene deletion and complemented strains. The gpaB ORF was confirmed by primers gpaB/OF and gpaB/OR; fragment AP was confirmed by primers gpaB/p1 and P801; while fragment APC was confirmed by primers gpaB/p1 and ptrA/R; (C) RT-PCR verification of gpaB gene deletion. The β-tubulin gene was used as a reference.
