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. 2018 Mar 10;10(3):117. doi: 10.3390/toxins10030117

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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gpaB is involved in the regulation of intracellular cAMP levels and PKA activities in A. flavus. (A) Deletion of gpaB leads to decreased accumulation of cAMP levels in A. flavus; (B) Disruption of gpaB leads to a change of the cAMP-dependent protein kinase activities in A. flavus. A 0.8% agarose gel was used to separate protein samples. Phosphorylated peptides migrated towards the cathode (+), while nonphosphorylated peptides migrated towards the anode (−). P, phosphorylated sample control; N, non-phosphorylated sample control. The triple asterisks “***” represent significant differences compared to the wildtype according to the t-test with p < 0.001. The experiments were conducted with three replicates for the indicated strain and were repeated three times.