Table 1.
Expression levels of genes involved in asexual development and AF biosynthesis analyzed by RT-qPCR.
| Gene ID | Name | Relative Expression Level | |
|---|---|---|---|
| WT | ΔgpaB | ||
| AFLA_029620 | abaA | 1.00 ± 0.02 | 0.17 ± 0.01 ** |
| AFLA_082850 | brlA | 1.00 ± 0.05 | 0.09 ± 0.01 ** |
| AFLA_131330 | nsdC | 1.21 ± 0.13 | 4.28 ± 0.21 *** |
| AFLA_020210 | nsdD | 0.86 ± 0.05 | 2.91 ± 0.11 ** |
| AFLA_040260 | sclR | 1.18 ± 0.18 | 7.20 ± 0.87 *** |
| AFLA_139360 | aflR | 0.88 ± 0.04 | 0.36 ± 0.03 *** |
| AFLA_139340 | aflS | 0.91 ± 0.09 | 0.26 ± 0.03 *** |
| AFLA_139210 | aflP | 1.11 ± 0.17 | 0.04 ± 0.0003 *** |
Genes’ levels were normalized to the house keeping gene actin and calculated by the 2−ΔΔCT method. Asterisks “**” and “***” represent significant differences compared to the wildtype for each gene according to the t-test with p < 0.01 and p < 0.001, respectively. The experiments were conducted with three replicates for the indicated strain and were repeated three times.