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. 2018 Mar 12;10(3):120. doi: 10.3390/toxins10030120

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of Aspergillus carbonarius alb1 transformants. (a) Map of plasmid pRF-HU2_alb1. (b) Transformation plate containing colonies displaying fawn pigmented conidia (putative Δalb1) or wild-type-like morphology regarding conidia pigmentation. (c) Diagram of the wild-type locus and the alb1 replacement with the hygromycin resistant selectable marker from pRF-HU2_alb1 by homologous recombination to generate the Δalb1 mutants. Primers used in the construction of plasmid pRF-HU2_alb1 and those used for the analysis of the transformants are shown. (d) Polymerase chain reaction (PCR) analysis of the wild-type AC49 strain and three knockout transformants (Δalb1-1, Δalb1-2, Δalb1-3).