Table 3.
Total OTA produced by the Δalb1 mutants and the WT strain of A. carbonarius in 4-mm-diam agar plugs with mycelium and conidia after 7 days of incubation.
| Strain | OTA (ng/mm2 ± Standard Error) | ||
|---|---|---|---|
| MM * | PDA | CA | |
| 25 °C | |||
| WT | 11.59 ± 0.67 B | 87.89 ± 2.39 B | 296.00 ± 22.41 B |
| Δalb1-1 | 90.15 ± 12.73 A (677.8) ** | 124.78 ± 1.19 A (41.9) | 492.26 ± 34.18 A (66.3) |
| Δalb1-2 | 85.81 ± 7.00 A (640.4) | 116.46 ± 3.39 A (32.5) | 503.45 ± 13.59 A (70.1) |
| Δalb1-3 | 99.21 ± 6.71 A (755.9) | 119.69 ± 5.75 A (36.2) | 550.03 ± 41.31 A (85.5) |
| 30 °C | |||
| WT | 12.03 ± 0.55 B | 28.80 ± 1.14 B | 47.11 ± 2.92 B |
| Δalb1-1 | 44.41 ± 5.28 A (269.2) | 50.34 ± 1.33 A (70.8) | 126.68 ± 5.40 A (168.9) |
| Δalb1-2 | 43.97 ± 0.98 A (265.5) | 48.35 ± 1.82 A (67.9) | 117.97 ± 4.90 A (150.4) |
| Δalb1-3 | 39.37 ± 1.19 A (227.2) | 43.08 ± 2.64 A (49.6) | 112.08 ± 12.43 A (137.9) |
Letters refer to comparisons within the same medium for each temperature (Tukey’s test at probability level p ≤ 0.01). * MM: Minimal Medium; PDA: Potatoes Dextrose Agar; CA: Coconut Agar. ** In parenthesis: percentage of OTA increase as compared to the wild type calculated with the formula [(OTAΔalb1 − OTAWT)/OTAWT] × 100.