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HilE does not disrupt HilD dimerization. A bacterial monohybrid system with the indicated genes fused to a wild-type lexA DNA binding domain were grown under SPI1-inducing conditions with the indicated concentrations of IPTG. Where indicated, hilE was overexpressed from pWKS30 (n = 6, 3 sets of duplicate assays on 3 separate days; error bars represent one standard deviation). The strains used were SU101 with pSR658, pDD506, pSR658::hilD, or pSR658::hilC with and without pHilE.
