FIG 7.

HilE regulates HilD activity independently of stability. The hilD-3XFLAG construct is expressed from a tetA promoter. Strains with the indicated genotypes were grown to mid-log phase and induced with tetracycline. Western blotting and β-galactosidase assay were performed using aliquots from the same sample. Densitometry of the Western blot was performed using ImageJ. HilD levels and β-galactosidase activity were normalized to those of the hilE⁺ strain. The strains used were JS2108, JS2109, JS2110, JS2111, JS2112, and JS2113.