FIG 3.

(A and B) PceA activity (A) and protein level (B) in crude extracts derived from cells grown with PCE in the presence of different benzimidazoles (25 μM). For the immunological analysis, 5 μg of protein was applied to each lane. The immunoblot was developed with an antiserum containing polyclonal antibodies against PceA. The data for the PceA activity were obtained from three different cultures. The standard deviation is given. prePceA, precursor with the N-terminal Tat signal peptide; PceA, processed form without the Tat signal peptide. (C) PCE-dependent growth of S. multivorans in the presence of 5-MeBza or DMB. Cells were harvested for functional analyses in the growth phase indicated by the dashed line.