FIG 1.

Genomic organization of the silC gene and generation of the ΔsilC mutant of F. tularensis. (A) Genomic organization of the silC gene of F. tularensis LVS. The thin arrows and the arrowheads indicate the location of the flanking primers and primers internal to the silC gene, respectively, used for the confirmation of silC gene deletion. (B and C) Confirmation of silC gene deletion by PCR using flanking primers (B) and primers internal to the silC gene (C). sodB gene-specific primers were used as internal controls.