FIG 9.

Relative gene expression of putative CcpA-dependent genes. Gene expression is shown as fold change of cells cultured with 2% glucose normalized to growth at 0.3% glucose. Standard deviations are indicated by error bars. spxB, pyruvate oxidase (SSA_0391; SGO_0292); eno, enolase gene (SSA_0886; SGO_1426); ldh, l-lactate dehydrogenase gene (SSA_1221; SGO_1232); ccpA, catabolite control protein A gene (SSA_1576; SGO_0773); scrA, gene for phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine specific (SSA_0456, SGO_1857); pykF, pyruvate kinase gene (SSA_0848; SGO_1339); manL, gene for phosphotransferase system, mannose-specific EIIAB (SSA_1918; SGO_1679). Genes were selected based on the RegPrecise transcription factor database (37). Cells were grown overnight in BHI and washed in PBS, and the A₆₀₀ was adjusted to 0.5. Four milliliters of BHI with or without the addition of glucose was inoculated with 350 μl of PBS-washed cells and aerobically incubated (200 rpm, 37°C). After 5 h of incubation, the RNA was isolated and quantitative PCR was performed. All experiments were performed in two technical and three biological replicates. Significant differences are indicated by asterisks (two-tailed paired t test; **, P < 0.01).