TABLE 1.
CodY mediated regulation of saeP-GFP fusions
| Strain | Genotype | RFUa | Fold regulationb |
|---|---|---|---|
| Strains expressing the saeP235p+-gfp fusion | |||
| SRB1008 | Wild type | 1,663 ± 335 | |
| SRB1009 | ΔcodY | 8,991 ± 1,172** | 5.4 |
| SRB1042 | Δrot | 3,448 ± 333 | |
| SRB1043 | Δrot ΔcodY | 16,530 ± 402** | 4.8 |
| Strains expressing the saeP235pscrambled-gfp fusion | |||
| SRB1162 | Wild type | 2,086 ± 212 | |
| SRB1163 | ΔcodY | 3,442 ± 170 | 1.6 |
| SRB1164 | Δrot | 2,367 ± 731 | |
| SRB1165 | Δrot ΔcodY | 5,108 ± 992* | 2.2 |
a
Relative fluorescence units (RFU) were determined by using the measured fluorescence values normalized to the OD600 value and subtracting the background value of cells lacking the fusion. Reported values (means ± standard errors of the means) are the results of three independent experiments. Values were compared to those of SRB1008, and significance was determined by one-way ANOVA with Tukey's multiple-comparison test (*, P < 0.01; **, P < 0.001).
b
Fold regulation is reported as RFUΔcodY mutant/RFUwild type or RFUΔcodY Δrot mutant/RFUΔrot mutant, where the subscript indicates the relevant strain.