TABLE 3.

List of primers used in the study

Primer by function	Sequence (5′–3′)
Deletion mutation and reverse complementation constructs
PepO-5U-F	TAGAGAATTCCTACCAACGCTTGATTGCTTTGCG
PepO-5U-R	CCATAAAACTAAAGTCGTATCTCCTCGTATCTCCTTATATCATTTTAAATTTGTAGAATCTTTATC
PepO-3D-F	TTCATTCTAATTGGTAATCAGTTAGGTTAGGTAGTTGATAAACCTTCAAATGCCTGTGAT
PepO-3D-R	ATAGCTCGAGAAGCTCAATTATCAGACGATGTGATTACTG
Spec-F	CCTAACTGATTACCAATTAGAATGAATATTTCCCAAATATTAAATAATAAAAC
Spec-R	GGAGATACGACTTTAGTTTTATGGAAATGAAAGATCATATCATATATAATCTAG
Protein expression studies
pET151PepO-F	CACCATGACAACTTATCAAGATGAT
pET151PepO-R	TTACCAAATAATCACACGGTCTTTCGG
qPCR studies
gyrA-F	CGACTTGTCTGAACGCCAAA
gyrA-R	GTCAGCAATCAAGGCCAACA
pepO-F	TGCCTTTAAAGAGCGCACAG
pepO-R	CTACCCCACCTAGATCAGCG
speB-F	TGCTGACGGACGTAACTTCT
speB-R	CCACCAGTACCAAGAGCTGA
aroE.2-F	ACAGTTGTATACAGGGAGCCA
aroE.2-R	ATTTGTTGATCAGCCAATTCCTT
ropB-F	TGAACGGTGTTGTGTGTCTT
ropB-R	TCTGCACACACTTTTGCTCA