. 2018 Feb 28;10(3):105. doi: 10.3390/v10030105

Table 1.

Alphavirus nsP3-host interactions. The table summarizes cellular proteins reported to interact with alphavirus nsP3. Viruses and cell types involved in each study are indicated. For methodology please refer to the original publications. Cell types: BHK-21—Baby hamster kidney fibroblasts; C₇10—Aedes albopictus; HEK293—Human embryonic kidney; 293T—HEK293 expressing SV40 large T antigen; 293FT—Fast-growing, highly transfectable clonal isolate derived of 293T; HeLa—Human cervix epitheloid carcinoma; MEF—Mouse embryonic fibroblasts; N2A—Mouse neuroblastoma; NIH 3T3—Mouse embryonic fibroblasts; NSC34—Mouse motor neuron-like hybrid; Rat2—Rattus norvegicus fibroblasts; Sf21—Spodoptera frugiperda ovarian cells; U87MG—Human astrocytoma cells.

Host Protein Interacting with nsP3	Cell Type	Virus	Ref.
14-3-3 z	C₇10	SINV	[18]
14-3-3 β/α	BHK-21	SINV	[60]
14-3-3 γ	BHK-21	SINV	[60]
14-3-3 ε	Rat2, HEK293	SINV	[46]
	BHK-21	SINV	[60]
	C₇10	SINV	[18]
14-3-3 ζ	Rat2, HEK293	SINV	[46]
14-3-3 ζ/δ	BHK-21	SINV	[60]
14-3-3 η	Rat2, HEK293	SINV	[46]
14-3-3 τ	BHK-21	SINV	[60]
Amphiphysin-1	In vitro, 293FT, N2A	SFV, SINV, CHIKV	[62]
Amphiphysin-2/BIN1—Bridging integrator 1	In vitro	CHIKV, SFV	[69]
	BHK-21	SINV	[67]
	In vitro, 293FT, HeLa	SFV, SINV, CHIKV	[62]
	NIH 3T3	CHIKV, SINV	[66]
B23.2	BHK-21	SINV	[60]
Beta-actin	BHK-21	SINV	[60]
Beta-tubulin	BHK-21	SINV	[60]
CAPZA1—F-actin-capping protein subunit alpha-1	BHK-21	VEEV	[67]
	NIH 3T3	EEEV, VEEV	[66]
CAPZA2—F-actin-capping protein subunit alpha-2	NIH 3T3	EEEV, VEEV	[66]
CAPZB—F-actin-capping protein subunit beta	BHK-21	VEEV	[67]
	NIH 3T3	EEEV, VEEV	[66]
CD2AP/CMS—CD2-associated protein	BHK-21	VEEV	[67]
	In vitro	SFV, SINV, CHIKV	[62]
	NIH 3T3	EEEV, VEEV, CHIKV	[66]
CKAP4—Cytoskeleton-associated protein 4	Rat2	SINV	[46]
CLTC—Clathrin heavy chain	NIH 3T3	EEEV	[66]
DDX1—DEAD box polypeptide 1	U87MG	VEEV	[70]
DDX3—DEAD/H box polypeptide 3	U87MG	VEEV	[70]
DDX5—DEAD box polypeptide 5	Rat2	SINV	[46]
	NIH 3T3	SINV	[66]
DDX6—DEAD box polypeptide 6	NIH 3T3	SINV	[66]
DDX17—DEAD box polypeptide 17	Rat2	SINV	[46]
	NIH 3T3	SINV	[66]
Desmin	BHK-21	SINV	[60]
DHX9—DEAD/H Box Polypeptide 9/ATP-dependent RNA helicase A	Rat2	SINV	[46]
DNAJC9—DnaJ heat shock protein family (Hsp40) member C9	NIH 3T3	CHIKV, SINV	[66]
Dnmt1—DNA (cytosine-5-)-methyltransferase 1	Rat2	SINV	[46]
eEF1A—Eukaryotic translation elongation factor 1A	BHK-21	SINV	[60]
eEF2—Eukaryotic translation elongation factor 2	Rat2	SINV	[46]
FHL2—Four and a half LIM domains protein 2	NIH 3T3	CHIKV	[66]
FMR1	BHK-21	VEEV	[67]
	NIH 3T3	EEEV, VEEV	[66]
FXR1	U87MG	VEEV	[70]
	BHK-21	VEEV	[67]
	BHK-21	SINV	[60]
	NIH 3T3	EEEV, VEEV	[66]
FXR2	BHK-21	VEEV	[67]
	NIH 3T3	EEEV, VEEV	[66]
G3BP-1/Rasputin	BHK-21	SINV	[60]
	Rat2, HEK293	SINV	[46]
	BHK-21	SINV	[67]
	BHK-21, MEF, HEK293	SFV	[53]
	In vitro	SFV	[52]
	BHK-21	SINV	[18]
	NIH 3T3	EEEV, CHIKV, SINV	[66]
	Sf21	CHIKV	[71]
	C₇10	SINV	[18]
G3BP-2	BHK-21	SINV	[67]
	BHK-21, MEF	SFV	[53]
	BHK-21	SINV	[18]
	NIH 3T3	EEEV, CHIKV, SINV	[66]
	Rat2, HEK293	SINV	[46]
GRP78—78 kDa glucose-regulated protein	Rat2	SINV	[46]
	C₇10	SINV	[18]
GSTM1—Glutathione S-transferase Mu 1	Rat2	SINV	[46]
HELQ—ATP-dependent DNA helicase Hel308	Rat2	SINV	[46]
HIST1H1C—Histone cluster 1 H1 family member C	NIH 3T3	CHIKV	[66]
hnRNP A0	BHK-21	SINV	[60]
hnRNP A1	Rat2	SINV	[46]
	BHK-21	SINV	[60]
hnRNP A1-like	U87MG	VEEV	[70]
hnRNP A2/B1	Rat2	SINV	[46]
	BHK-21	SINV	[60]
hnRNP A3	Rat2	SINV	[46]
	BHK-21	SINV	[60]
hnRNP C	HeLa	SFV	[61]
hnRNP G	Rat2	SINV	[46]
hnRNP K	HeLa	SFV	[61]
	HeLa, BHK-21	SINV	[72]
hnRNP M	HeLa	SFV	[61]
hnRNP U	BHK-21	SINV	[60]
HSC70—Heat shock cognate 71kDa protein	Rat2	SINV	[46]
	BHK-21	SINV	[60]
	BHK-21, C₇10	SINV	[18]
HSP90—Heat shock protein 90	293T	CHIKV	[73]
HSPA1B—Heat shock protein 1B	NIH 3T3	EEEV, VEEV, CHIKV, SINV	[66]
IGF2BP1—Insulin-like growth factor 2 mRNA-binding protein 1	NIH 3T3	EEEV, SINV	[66]
IGF2BP2	NIH 3T3	EEEV, SINV	[66]
IGF2BP3	NIH 3T3	EEEV, VEEV, CHIKV, SINV	[66]
IKKβ—Inhibitor of nuclear factor kappa-B kinase subunit beta	U87MG	VEEV, WEEV	[65]
INTS6—Integrator Complex Subunit 6/RNA helicase HDB/DICE1	Rat2	SINV	[46]
JUN—Jun proto-oncogene	NIH 3T3	CHIKV	[66]
Lysophospholipase 1,2	C₇10	SINV	[18]
MAP 1B—Microtubule-associated protein 1B	U87MG	VEEV	[70]
MYBBP1A—MYB binding protein 1A	NIH 3T3	CHIKV, SINV	[66]
MYH9—Myosin Heavy Chain 9	BHK-21	SINV	[60]
Myosin regulatory light chain	BHK-21	SINV	[60]
NAP1L1—Nucleosome assembly protein 1-like 1	NIH 3T3	CHIKV	[66]
NAP1L4—Nucleosome assembly protein 1-like 4	NIH 3T3	CHIKV	[66]
Nucleolin	Rat2	SINV	[46]
p85—PI3K subunit p85	HEK293, BHK-21	SFV, RRV	[63]
PARP-1—Poly(ADP-ribose) polymerase-1	NSC34, 293T	SINV	[64]
PCBP1/hnRNP E1—Poly(rC)-binding protein 1	HeLa	SFV	[61]
Peripherin 1	Rat2	SINV	[46]
PGAM5—PGAM family member 5, serine/threonine phosphatase	NIH 3T3	EEEV, VEEV, CHIKV	[66]
Plectin 1	BHK-21	SINV	[60]
	U87MG	VEEV	[70]
PSF—PTB-associated splicing factor	Rat2	SINV	[46]
RACK1/GNB2L1—Receptor of activated protein C kinase 1/Guanine nucleotide-binding protein subunit beta-2-like 1	Rat2	SINV	[46]
RDX—Radixin	NIH 3T3	CHIKV	[66]
Ribosomal protein L10	BHK-21	SINV	[60]
Ribosomal protein L10A	U87MG	VEEV	[70]
	BHK-21	SINV	[60]
	C₇10	SINV	[18]
Ribosomal protein L15	Rat2	SINV	[46]
Ribosomal protein L18A	Rat2	SINV	[46]
Ribosomal protein L23A	C₇10	SINV	[18]
Ribosomal protein L3	Rat2	SINV	[46]
Ribosomal protein L4	C₇10	SINV	[18]
Ribosomal protein L5	BHK-21	SINV	[18]
Ribosomal protein L6	U87MG	VEEV	[70]
	BHK-21	SINV	[18]
Ribosomal protein L7	BHK-21	SINV	[60]
	C₇10	SINV	[18]
Ribosomal protein L7A	BHK-21	SINV	[18]
Ribosomal protein L8	C₇10	SINV	[18]
Ribosomal protein P0	U87MG	VEEV	[70]
Ribosomal protein S10	Rat2	SINV	[46]
Ribosomal protein S18	BHK-21	SINV	[60]
Ribosomal protein S3	Rat2	SINV	[46]
Ribosomal protein S8	U87MG	VEEV	[70]
	Rat2	SINV	[46]
Ribosomal protein S9	Rat2	SINV	[46]
	BHK-21	SINV	[60]
S100A4—S100 calcium binding protein A4	NIH 3T3	EEEV, VEEV	[66]
SASH1—SAM and SH3 domain-containing protein 1	In vitro	SFV, SINV, CHIKV	[62]
SH3BP-5—SH3-domain binding protein 5	Rat2	SINV	[46]
SH3KBP1/CIN85—SH3 domain-containing kinase-binding protein 1	In vitro	SFV, SINV, CHIKV	[62]
	NIH 3T3	EEEV, VEEV, CHIKV	[66]
SK2—Sphingosine kinase 2	HeLa	CHIKV	[74]
SLC25A13—Solute carrier family 25 member 13	NIH 3T3	EEEV, VEEV	[66]
SLC25A5—Solute carrier family 25 member 5/Adenine nucleotide translocator 2	NIH 3T3	EEEV, VEEV	[66]
	Rat2	SINV	[46]
SNX33—Sorting nexin-33	NIH 3T3	EEEV	[66]
SNX9—Sorting nexin-9	NIH 3T3	EEEV	[66]
TRBP—TAR RNA-binding protein	C₇10	SINV	[18]
Vimentin	BHK-21	SINV	[60]
WDR48—WD repeat-containing protein 48	BHK-21	SINV	[67]
	NIH 3T3	SINV	[66]
YBX1—Y-box-binding protein 1	BHK-21	SINV	[60]
	BHK-21	SINV	[18]
	NIH 3T3	EEEV	[66]
YBX3—Y-box-binding protein 3	NIH 3T3	CHIKV, SINV	[66]