Table 1.
Gene | Sequence (5′-3′) Forward | Sequence (5′-3′) Reverse | Accession No. a |
---|---|---|---|
TLR4 | CTCTGCCTTCACTACAGAGA | CTGAGTCGTCTCCAGAAGAT | AB078418 |
TLR9 | GTGGAACTGTTTTGGCATC | CACAGCACTCTGAGCTTTGT | AB071394 |
TGF-β | CACGTGGAGCTATACCAGAA | TCCGGTGACATCAAAGGACA | Y00111 |
TNF-α | ATTCAGGGATGTGTGGCCTG | CCAGATGTCCCAGGTTGCAT | JF831365.1 |
β-actin | CATCACCATCGGCAACGA | GCGTAGAGGTCCTTCCTGATGT | U07786 |
PEDV-M | GGTTCTATTCCCGTTGATGAGGT | AACACAAGAGGCCAAAGTATCCAT | AF353511.1 |
a The sequences of the two primers were checked using NCBI BLAST Software (NCBI, Bethesda, MD, USA), and no significant alignment with any other animal virus gene was found.