Figure 5.

No vector-neutralizing antibodies were induced in mice after repeated VSV-GP injection. C57BL6 mice were immunized either three times (weeks 0, 8, and 16), and plasma was collected 23 weeks after the first immunization, or mice were immunized eight times within 40 weeks (weeks 0, 2, 4, 6, 8, 10, 14, and 40) and plasma was collected 42 weeks after the first immunization. Plasma pools from four vaccinated animals were pre-incubated with the replication incompetent VSV*ΔG-GP virus and subsequently BHK-21 were infected. As a negative control, plasma from a naïve mouse was analyzed. As a positive control, the lymphocytic choriomeningitis virus (LCMV)-neutralizing mouse monoclonal antibody Wen4 was used. The 1:10 dilution from the naïve mouse was set to 1. The graph shows the mean ± SEM of triplicate samples.