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. 2018 Mar 7;10(3):115. doi: 10.3390/v10030115

Table 5.

Expression variations for cII in 594 host.

CII-Construct Plasmids with Thermally Inducible Expression of cII Allele Intensity of CII-Activated CI434 Expression at 37–39 °C a λimm434 cII Plaque Formation at 37–39 °C a Cell Viability (±SE) [% Plasmid Loss] Per Growth Temp. of Transformants
39 °C 42 °C
sR-38339-pE-cII (p747) H 0.62 (0.10) [0] <0.001 (<0.004) [94]
sR-38339-pE-cII-oop-pO94 (p748) 0 + d 0.82 (0.02) [0] 0.001 (<0.0001) [94]
sR-38339-pE-cII-cy3048 in −10 pE (p767) 0-S b + 0.89 (0.01) [0] 0.08 (<0.0001) [0]
sR-38339-pE-cII-cy2001 in −10 pE (p765) 0-S b + 0.69 (<0.0001) [0] 0.01 (<0.0001) [0]
sR-38339-pE-cII-cy42 in −35 pE (p764) 0-S b + 1.00 (0.020) [0] 1.00 (<0.0001) [42]
sR-38339-pE-cII-cy3001 in −35 pE (p766) H 0.72 (<0.0001) [0] <0.001 (0.001) [8]
cII-oop-pO45WT (p763) H 0.72 (0.10) [0] 0.07 (<0.0001) [0]
cII-oop-pO45-38,683-87MH2 in −10 pO (p759) H 0.67 (0.10) [0] <0.001 (0.002) [100]
cII-oop-pO45-38683LK in −10 pO (p762) H 0.78 (0.20) [0] <0.001 (<0.0001) [50]
cII-oop-pO94-38683-87MH (p681) c H 0.71 (<0.0001) [0] <0.001 (<0.0001) [86]

a Designations as in Table 2. b 0-S = Phage lysis spots/plaque areas were weak-turbid at 39 °C but well-formed clear plaques were observed at 37 °C. c Sequence change determined in putative −10 region of pO recovered from p27RpO [39] was attatG to cgcgcG and was identical to the synthetic change introduced into p759, see Figure S1, plasmids 2. d Individual plaques were clear.