Table 5.
CII-Construct Plasmids with Thermally Inducible Expression of cII Allele | Intensity of CII-Activated CI434 Expression at 37–39 °C a | λimm434 cII− Plaque Formation at 37–39 °C a | Cell Viability (±SE) [% Plasmid Loss] Per Growth Temp. of Transformants | |
---|---|---|---|---|
39 °C | 42 °C | |||
sR-38339-pE-cII (p747) | H | − | 0.62 (0.10) [0] | <0.001 (<0.004) [94] |
sR-38339-pE-cII-oop-pO94 (p748) | 0 | + d | 0.82 (0.02) [0] | 0.001 (<0.0001) [94] |
sR-38339-pE-cII-cy3048 in −10 pE (p767) | 0-S b | + | 0.89 (0.01) [0] | 0.08 (<0.0001) [0] |
sR-38339-pE-cII-cy2001 in −10 pE (p765) | 0-S b | + | 0.69 (<0.0001) [0] | 0.01 (<0.0001) [0] |
sR-38339-pE-cII-cy42 in −35 pE (p764) | 0-S b | + | 1.00 (0.020) [0] | 1.00 (<0.0001) [42] |
sR-38339-pE-cII-cy3001 in −35 pE (p766) | H | − | 0.72 (<0.0001) [0] | <0.001 (0.001) [8] |
cII-oop-pO45WT (p763) | H | − | 0.72 (0.10) [0] | 0.07 (<0.0001) [0] |
cII-oop-pO45-38,683-87MH2 in −10 pO (p759) | H | − | 0.67 (0.10) [0] | <0.001 (0.002) [100] |
cII-oop-pO45-38683LK in −10 pO (p762) | H | − | 0.78 (0.20) [0] | <0.001 (<0.0001) [50] |
cII-oop-pO94-38683-87MH (p681) c | H | − | 0.71 (<0.0001) [0] | <0.001 (<0.0001) [86] |
a Designations as in Table 2. b 0-S = Phage lysis spots/plaque areas were weak-turbid at 39 °C but well-formed clear plaques were observed at 37 °C. c Sequence change determined in putative −10 region of pO recovered from p27RpO− [39] was attatG to cgcgcG and was identical to the synthetic change introduced into p759, see Figure S1, plasmids 2. d Individual plaques were clear.