Figure 6.

Knockdown of hnRNP A1 expression inhibits YN13 replication. CCL-81 cells were transfected without siRNA (a) or with NC (b) or siRNA-1 (c). At 60 hpt, cells were infected with YN13 Strain at a moi of 0.001 or mock infected (d). Virus suspensions were harvested at 24 hpi to calculate the virus titer by real-time RT-PCR. The virus copies per mL suspension was calculated (A). Cells were harvested at 24 hpi to analyze the expression of PEDV N protein by Western blot (B) and the numbers of cells infected with PEDV by IFA (Original magnification 100×) (C). PEDV N protein levels were quantified by measuring band intensities and normalized with respect to the amount of β-actin. Data are shown as means ± SEM of at least three independent experiments, with the error bars representing the standard deviations.