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. 2018 Mar 27;8:5239. doi: 10.1038/s41598-018-23575-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic distribution and main events in the evolution of the Rheb protein. The schematic phylogeny shown is a consensus of recent molecular phylogenies with the last eukaryotic common ancestor (LECA) based on ref.⁵⁴. Note that alternative LECA positions suggested by other authors^55–58 are all also compatible with Rheb being present already in the LECA. Species representatives for each clade are listed in Supplementary Table S1. A detailed view of the Rheb evolution in Euglenozoa and the SAR clade is provided in Figs 3A and 4, respectively. Only two Rheb-lacking lineages of Saccharomycotina are shown for simplicity; for a more comprehensive view of the Rheb distribution in this group see ref.⁸.