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. 2018 Mar 27;8:5271. doi: 10.1038/s41598-018-23593-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of the expression cassettes used in this work. (a) Gag-Pro-Pol expression cassettes. (b) Rev expression cassettes. (c) Envelope expression cassettes. (d) LV genome expression cassettes. Abbreviations: CMV, Cytomegalovirus promoter; Int, intron; GPP, gag-pro-pol sequence; pAn, polyA sequence; GP(T26S)P, gag-pro-pol with mutated T26S protease sequence; Blast^R, blasticidin resistance gene; RSV, Rous Sarcoma Virus promoter; Hygro^R, hygromycin resistance gene; WPRE, woodchuck hepatitis post-transcriptional regulatory element; VSV-G, glycoprotein G of the vesicular stomatitis virus; SV40/FerH, ferritin heavy chain (FerH) promoter fused with SV40 enhancer; IRES, internal ribosome entry site; Zeo^R, zeocin resistance gene; Ψ, packaging signal sequence; hPGK, human phosphoglycerate kinase promoter; Puro^R, puromycin resistance gene. The thinner grey arrows represent the mRNA transcripts. The filled grey boxes represent the spacer region that drives the re-initiation of the translation mechanism.